Difference between revisions of "Part:BBa K4286099"
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| − | 2022 SZU-China has designed | + | 2022 SZU-China has designed a timed suicide switch mediated by a classical synthetic gene oscillator. In the negative feedback loop, the oscillator is composed of three genes encoding repressor protein -- TetR from the Tn10 transposon, cI from bacteriophage lambda, and LacI from the lactose operon -- each repressor protein is attached to an LVA degradation tag located at the carboxy terminus. The concentration of each kind of repressor protein changed cyclically: lacI inhibited the expression of tetR, tetR inhibited the expression of cI, and cI inhibited lacI expression. |
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Revision as of 14:09, 9 October 2022
Classical oscillator composed of three genes encoding repressor protein
2022 SZU-China has designed a timed suicide switch mediated by a classical synthetic gene oscillator. In the negative feedback loop, the oscillator is composed of three genes encoding repressor protein -- TetR from the Tn10 transposon, cI from bacteriophage lambda, and LacI from the lactose operon -- each repressor protein is attached to an LVA degradation tag located at the carboxy terminus. The concentration of each kind of repressor protein changed cyclically: lacI inhibited the expression of tetR, tetR inhibited the expression of cI, and cI inhibited lacI expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2188
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]