Difference between revisions of "Part:BBa K4414007"

 
Line 1: Line 1:
  
 
__NOTOC__
 
__NOTOC__
 +
 
<partinfo>BBa_K4414007 short</partinfo>
 
<partinfo>BBa_K4414007 short</partinfo>
  
−
Report gene.Engineered red fluorescent protein
+
==Usage and Biology==
  
 +
Tandem-dimer tomato (tdTomato) is a stable, robust red fluorescent protein that was modified from previous fluorescence proteins in order to have enhanced fluorescence properties.[1]
 +
 +
 +
===Sequecing===
 +
The plasmid was sequenced correct.
  
−
<!-- Add more about the biology of this part here
 
−
===Usage and Biology===
 
  
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
===Sequence and Features===
 
<partinfo>BBa_K4414007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4414007 SequenceAndFeatures</partinfo>
  
Line 18: Line 22:
 
<partinfo>BBa_K4414007 parameters</partinfo>
 
<partinfo>BBa_K4414007 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
==Functional Validation and result==
 +
 +
 +
We design plasmids to produce tdTomato, and the pictures below are the fluorescent protein image of the pigment protein in the cell and the cell clump containing the pigment protein under the hydrocoagulant membrane.
 +
 +
<html>
 +
 +
<figure class="figure">
 +
<img src="https://static.igem.wiki/teams/4414/wiki/016-4.jpg" class="figure-img img-fluid rounded"  height="250px">
 +
 +
</figure>
 +
 +
</html>
 +
Figure 2.The picture on the left is Bright-field cell diagram, the picture in the middle is fluorescence diagram, and the picture on the right is merge diagram.
 +
 +
===Reference===
 +
[1].Syverud, B. C., Gumucio, J. P., Rodriguez, B. L., Wroblewski, O. M., Florida, S. E., Mendias, C. L., & Larkin, L. M. (2018). A Transgenic tdTomato Rat for Cell Migration and Tissue Engineering Applications. Tissue Engineering. Part C, Methods, 24(5), 263–271. https://doi.org/10.1089/ten.TEC.2017.0406

Revision as of 13:29, 9 October 2022


tdTomato

Usage and Biology

Tandem-dimer tomato (tdTomato) is a stable, robust red fluorescent protein that was modified from previous fluorescence proteins in order to have enhanced fluorescence properties.[1]


Sequecing

The plasmid was sequenced correct.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Validation and result

We design plasmids to produce tdTomato, and the pictures below are the fluorescent protein image of the pigment protein in the cell and the cell clump containing the pigment protein under the hydrocoagulant membrane.

Figure 2.The picture on the left is Bright-field cell diagram, the picture in the middle is fluorescence diagram, and the picture on the right is merge diagram.

Reference

[1].Syverud, B. C., Gumucio, J. P., Rodriguez, B. L., Wroblewski, O. M., Florida, S. E., Mendias, C. L., & Larkin, L. M. (2018). A Transgenic tdTomato Rat for Cell Migration and Tissue Engineering Applications. Tissue Engineering. Part C, Methods, 24(5), 263–271. https://doi.org/10.1089/ten.TEC.2017.0406