Difference between revisions of "Part:BBa K4343101"

 
 
Line 3: Line 3:
 
<partinfo>BBa_K4343101 short</partinfo>
 
<partinfo>BBa_K4343101 short</partinfo>
  
−
PaDes12 encodes &#8710;-12 desaturase from Parietichytrium sp. which functions in the desaturase and elongase pathway and could convert C18:1 to C18:2.
+
PaDes12 encodes ∆-12 desaturase from Parietichytrium sp. which functions in the desaturase and elongase pathway and could convert C18:1 to C18:2.
  
 +
===Description===
 +
The PaDes12 gene encodes a protein of 399 amino acids. Heterologous expression of PaDes12 could catalyze C18:1 to C18:2 in Y. lipolytica.
 +
 +
Yohei Ishibashi et al. applied this enzyme in C20ELO KO cells to obtain a 6-fold higher yield of the target product, demonstrating the high availability of this enzyme activity.
 +
 +
===Reference===
 +
Ishibashi, Y., Goda, H., Hamaguchi, R., Sakaguchi, K., Sekiguchi, T., Ishiwata, Y., ... & Ito, M. (2021). PUFA synthase-independent DHA synthesis pathway in Parietichytrium sp. and its modification to produce EPA and n-3DPA. Communications Biology, 4(1), 1-11.
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 11:41, 9 October 2022


PaDes12

PaDes12 encodes ∆-12 desaturase from Parietichytrium sp. which functions in the desaturase and elongase pathway and could convert C18:1 to C18:2.

Description

The PaDes12 gene encodes a protein of 399 amino acids. Heterologous expression of PaDes12 could catalyze C18:1 to C18:2 in Y. lipolytica.

Yohei Ishibashi et al. applied this enzyme in C20ELO KO cells to obtain a 6-fold higher yield of the target product, demonstrating the high availability of this enzyme activity.

Reference

Ishibashi, Y., Goda, H., Hamaguchi, R., Sakaguchi, K., Sekiguchi, T., Ishiwata, Y., ... & Ito, M. (2021). PUFA synthase-independent DHA synthesis pathway in Parietichytrium sp. and its modification to produce EPA and n-3DPA. Communications Biology, 4(1), 1-11. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1217