Difference between revisions of "Part:BBa K4212038:Design"
 
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===Design Notes===
 
===Design Notes===
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This part consists the chimera of CotZ and ChiS, in which we combined these parts into level 1 Golden Gate Construct. Our dry lab team predicted the functional model of this chimera and our wet lab intended to test the effectiveness of this model. We intended to compare the fungal-killing efficiency of CotZ and CotG chimera. Promoter hyper-spank is an inducible promoter, which was designed for high-level protein expression in B.subtilis and it was previously used by other iGEM teams.Our dry lab team predicted 2 possible chimeras, with either CotG or CotZ attached to the chitinase. Our wet lab team attempted to verify that the CotG chimera works better than the CotZ one. Promoter hyper-spank is an inducible promoter, which was designed for high-level protein expression in B.subtilis and it was previously used by other iGEM teams. The addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG) can induce gene expression under the control of the promoter hyperspank. tL3S2P21 is a strong promoter, which helps to increase the production of desired protein.
 
  
 +
This part consists the fusion protein consisted of CotZ and ChiS, in which we combined these parts into level 1 Golden Gate Construct. Our dry lab team predicted the functional model of this chimera and our wet lab intended to test the effectiveness of this model. We intended to compare the fungal-killing efficiency of CotZ and CotG fusion protein. Promoter hyper-spank is an inducible promoter, which was designed for high-level protein expression in B.subtilis and it was previously used by other iGEM teams.Our dry lab team predicted 2 possible chimeras, with either CotG or CotZ attached to the chitinase. Our wet lab team attempted to verify that the CotG chimera works better than the CotZ one. Promoter hyper-spank is an inducible promoter, which was designed for high-level protein expression in B.subtilis and it was previously used by other iGEM teams. The addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG) can induce gene expression under the control of the promoter hyperspank. tL3S2P21 is a strong terminator, which helps to increase the production of desired protein.
  
  

Latest revision as of 11:19, 9 October 2022


ChiS5


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1723
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1723
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1723
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part consists the fusion protein consisted of CotZ and ChiS, in which we combined these parts into level 1 Golden Gate Construct. Our dry lab team predicted the functional model of this chimera and our wet lab intended to test the effectiveness of this model. We intended to compare the fungal-killing efficiency of CotZ and CotG fusion protein. Promoter hyper-spank is an inducible promoter, which was designed for high-level protein expression in B.subtilis and it was previously used by other iGEM teams.Our dry lab team predicted 2 possible chimeras, with either CotG or CotZ attached to the chitinase. Our wet lab team attempted to verify that the CotG chimera works better than the CotZ one. Promoter hyper-spank is an inducible promoter, which was designed for high-level protein expression in B.subtilis and it was previously used by other iGEM teams. The addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG) can induce gene expression under the control of the promoter hyperspank. tL3S2P21 is a strong terminator, which helps to increase the production of desired protein.


Source

Synthetic construct.

References

https://parts.igem.org/Part:BBa_K143015