Difference between revisions of "Part:BBa K4212035:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| + | This part consists the fusion protein consisted of CotG and ChiS, in which we combined these parts into level 1 Golden Gate Construct. Our dry lab team predicted the functional model of this chimera and our wet lab intended to test the effectiveness of this model. Promoter hyper-spank is an inducible promoter, which was designed for high-level protein expression in B.subtilis and it was previously used by other iGEM teams. We added a flexible linker to this construct to test if it works better than the construct without the linker. | ||
===Source=== | ===Source=== | ||
| − | + | Synthetic construct. | |
===References=== | ===References=== | ||
| + | https://parts.igem.org/Part:BBa_K143015 | ||
Latest revision as of 11:16, 9 October 2022
ChiS2
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1903
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1903
Illegal NheI site found at 1011 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1903
Illegal BamHI site found at 1888 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1903
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1903
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part consists the fusion protein consisted of CotG and ChiS, in which we combined these parts into level 1 Golden Gate Construct. Our dry lab team predicted the functional model of this chimera and our wet lab intended to test the effectiveness of this model. Promoter hyper-spank is an inducible promoter, which was designed for high-level protein expression in B.subtilis and it was previously used by other iGEM teams. We added a flexible linker to this construct to test if it works better than the construct without the linker.
Source
Synthetic construct.