Difference between revisions of "Part:BBa K4387001"
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__NOTOC__
 
 
<partinfo>BBa_K4387001 short</partinfo>
 
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==Usage and Biology==
  
 
NorR is a transcriptional regulator of the nitric oxide sensitive promoter pNorV. This regulator was codon optimized for E.coli Nissle 1917 to avoid recombination. NorR acts as an activator of pNorV in the presence of nitric oxide and represses the expression of downstream genes otherwise[1].  
 
NorR is a transcriptional regulator of the nitric oxide sensitive promoter pNorV. This regulator was codon optimized for E.coli Nissle 1917 to avoid recombination. NorR acts as an activator of pNorV in the presence of nitric oxide and represses the expression of downstream genes otherwise[1].  
  
  
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===Sequence and Features===
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==Sequence and Features==
 
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===References===
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==References==
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<ul>
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Xiaoyu J. Chen, Baojun Wang, Ian P. Thompson, and Wei E. Huang et al. Rational Design and Characterization of Nitric Oxide Biosensors in E. coli Nissle 1917 and Mini SimCells ACS Synthetic Biology 2021 10 (10), 2566-2578 DOI: 10.1021/acssynbio.1c00223
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<li>[1] Xiaoyu J. Chen, Baojun Wang, Ian P. Thompson, and Wei E. Huang et al. Rational Design and Characterization of Nitric Oxide Biosensors in E. coli Nissle 1917 and Mini SimCells ACS Synthetic Biology 2021 10 (10), 2566-2578 <html><a href="https://pubs.acs.org/doi/abs/10.1021/acssynbio.1c00223">DOI: 10.1021/acssynbio.1c00223</a></html></li>
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</ul>
  
 
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Revision as of 09:17, 9 October 2022

Codon Optimized NorR

Usage and Biology

NorR is a transcriptional regulator of the nitric oxide sensitive promoter pNorV. This regulator was codon optimized for E.coli Nissle 1917 to avoid recombination. NorR acts as an activator of pNorV in the presence of nitric oxide and represses the expression of downstream genes otherwise[1].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  • [1] Xiaoyu J. Chen, Baojun Wang, Ian P. Thompson, and Wei E. Huang et al. Rational Design and Characterization of Nitric Oxide Biosensors in E. coli Nissle 1917 and Mini SimCells ACS Synthetic Biology 2021 10 (10), 2566-2578 DOI: 10.1021/acssynbio.1c00223