Difference between revisions of "Part:BBa K4488011"

 
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The construct can be cloned into an expression vector such as  pET28c in E.coli to produce a fusion protein of fuGFP with CBDclos. The fuGFP sequence is towards the N terminus of the protein with CBDclos (BBa_K1321341) downstream followed by a stop codon. Recognition sites for BamHI and BsaI are present before the RBS allowing golden gate cloning. XhoI and BsaI are also present downstream of the stop codon.  
 
The construct can be cloned into an expression vector such as  pET28c in E.coli to produce a fusion protein of fuGFP with CBDclos. The fuGFP sequence is towards the N terminus of the protein with CBDclos (BBa_K1321341) downstream followed by a stop codon. Recognition sites for BamHI and BsaI are present before the RBS allowing golden gate cloning. XhoI and BsaI are also present downstream of the stop codon.  
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Below is the fluorescence assay depicting a higher reading compared to our previous construct BBa_K4488007 :
 
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[TBA]
 
  
  
 
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===Usage and Biology===
 
===Usage and Biology===
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Below is the fluorescence assay depicting a higher reading compared to our previous construct BBa_K4488007 :
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[TBA]
  
 
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Revision as of 09:14, 9 October 2022


Fusion of free-use GFP with CBDclos (cellulose-binding domain) at the C-terminal end with a linker

The construct can be cloned into an expression vector such as pET28c in E.coli to produce a fusion protein of fuGFP with CBDclos. The fuGFP sequence is towards the N terminus of the protein with CBDclos (BBa_K1321341) downstream followed by a stop codon. Recognition sites for BamHI and BsaI are present before the RBS allowing golden gate cloning. XhoI and BsaI are also present downstream of the stop codon.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 163
  • 1000
    COMPATIBLE WITH RFC[1000]