Difference between revisions of "Part:BBa K274220"
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Amount of beta-carotene produced can be measured by photospectrometer with absorbance at 455nm (beta-carotene extraction using acetone). | Amount of beta-carotene produced can be measured by photospectrometer with absorbance at 455nm (beta-carotene extraction using acetone). | ||
| − | Datasheet for Part BBa_K274200, Part BBa_274210 and Part BBa_274220 in E.coli strain MG1655. You may also wish to refer to the "Experience | + | Datasheet for Part BBa_K274200, Part BBa_274210 and Part BBa_274220 in ''E. coli'' strain MG1655. You may also wish to refer to the "Experience" page. |
[[Image:Carotene datasheet1.JPG|200px]] | [[Image:Carotene datasheet1.JPG|200px]] | ||
Revision as of 02:23, 25 September 2009
CrtEBIY under pBad promoter
This Biobrick is created by putting enzyme cassette CrtEBIY (with individual rbs) of Part BBa_K274200 under arabinose-induced promoter I0500.
Enzyme cassette CrtEBIY (with individual rbs) of Part BBa_K274200 converts colourless farnesyl pyrophosphate to orange beta-carotene (via intermediates geranylgeranyl pyroiphosphate, phytoene and lycopene).
Amount of beta-carotene produced can be measured by photospectrometer with absorbance at 455nm (beta-carotene extraction using acetone).
Datasheet for Part BBa_K274200, Part BBa_274210 and Part BBa_274220 in E. coli strain MG1655. You may also wish to refer to the "Experience" page.
For PDF version of this Datasheet: File:Carotene.pdf
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
Illegal BamHI site found at 3192 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2728
Illegal NgoMIV site found at 2858
Illegal AgeI site found at 979
Illegal AgeI site found at 1943 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961