Difference between revisions of "Part:BBa K4156098"

 
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<partinfo>BBa_K4156098 short</partinfo>
 
<partinfo>BBa_K4156098 short</partinfo>
  
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pCadC-Bxb1is constructed with pH-sensitive promoter pCadC and serine integrase Bxb1.
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<!-- Add more about the biology of this part here -->
  
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===Usage and Biology===
 
===Usage and Biology===
  
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pCadC is regulated by the membrane lineage activator protein (CadC) and exhibits higher activity in acidic medium than in neutral pH medium. It is used in response to acidic conditions to allow the strain to rapidly localize to tumor cells and activate downstream gene transcription.Bxb1 is used to control downstream logic gate switching and to provide gene signaling amplification.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4156098 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4156098 SequenceAndFeatures</partinfo>

Revision as of 08:47, 9 October 2022


pCadC-Bxb1-φ174E

pCadC-Bxb1is constructed with pH-sensitive promoter pCadC and serine integrase Bxb1.


Usage and Biology

pCadC is regulated by the membrane lineage activator protein (CadC) and exhibits higher activity in acidic medium than in neutral pH medium. It is used in response to acidic conditions to allow the strain to rapidly localize to tumor cells and activate downstream gene transcription.Bxb1 is used to control downstream logic gate switching and to provide gene signaling amplification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 368
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 642
    Illegal XhoI site found at 729
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1476