Difference between revisions of "Part:BBa K4156081"

 
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<partinfo>BBa_K4156081 short</partinfo>
 
<partinfo>BBa_K4156081 short</partinfo>
  
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SpoVG is an endogenous ribosome binding site (RBS).
  
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===Usage and Biology===
 
===Usage and Biology===
  
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In order to obtain the highest level of translation from this RBS, it must be linked to a coding region preceded by a coding region. A standard prefix increases the distance between the RBS and the start codon and decreases the translation efficiency. In our experiments, we use hyper-spank in combination with spoVG and use this to form the pLldR subunit (<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_BBa_K4156101"> BBa_BBa_K4156101 </a></html>)
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4156081 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4156081 SequenceAndFeatures</partinfo>

Revision as of 08:30, 9 October 2022


RBS spoVG

SpoVG is an endogenous ribosome binding site (RBS).

Usage and Biology

In order to obtain the highest level of translation from this RBS, it must be linked to a coding region preceded by a coding region. A standard prefix increases the distance between the RBS and the start codon and decreases the translation efficiency. In our experiments, we use hyper-spank in combination with spoVG and use this to form the pLldR subunit ( BBa_BBa_K4156101 )

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]