Difference between revisions of "Part:BBa K4156078:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
To be refined
 
  
 +
It is mainly regulated by the transcriptional activator (FNR). In the absence of oxygen, the FNR binds to the [4Fe-4S]2+ cluster to generate a transcriptionally active homodimer. However, in the presence of oxygen, the [4Fe-4S]2+ cluster is degraded and the FNR dimer dissociates into inactive monomers.
  
  
 
===Source===
 
===Source===
  
−
To be refined
 
  
 
===References===
 
===References===
 +
<i>
 +
1 Yu B, Yang M, Shi L, et al. Explicit hypoxia targeting with tumor suppression by creating an
 +
"obligate" anaerobic Salmonella Typhimurium strain. Sci Rep. 2012;2:436. doi:10.1038/srep00436
 +
 +
 +
2 Crack JC, Jervis AJ, Gaskell AA, et al. Signal perception by FNR: the role of the iron-sulfur
 +
cluster. Biochem Soc Trans. Dec 2008;36(Pt 6):1144-8. doi:10.1042/bst0361144
 +
 +
 +
3 Goers L, Ainsworth C, Goey CH, Kontoravdi C, Freemont PS, Polizzi KM. Whole-cell
 +
Escherichia coli lactate biosensor for monitoring mammalian cell cultures during biopharmaceutical
 +
production. Biotechnol Bioeng. 2017;114(6):1290-1300. doi:10.1002/bit.26254
 +
</i>

Revision as of 08:27, 9 October 2022


-- No description --


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is mainly regulated by the transcriptional activator (FNR). In the absence of oxygen, the FNR binds to the [4Fe-4S]2+ cluster to generate a transcriptionally active homodimer. However, in the presence of oxygen, the [4Fe-4S]2+ cluster is degraded and the FNR dimer dissociates into inactive monomers.


Source

References

1 Yu B, Yang M, Shi L, et al. Explicit hypoxia targeting with tumor suppression by creating an "obligate" anaerobic Salmonella Typhimurium strain. Sci Rep. 2012;2:436. doi:10.1038/srep00436


2 Crack JC, Jervis AJ, Gaskell AA, et al. Signal perception by FNR: the role of the iron-sulfur cluster. Biochem Soc Trans. Dec 2008;36(Pt 6):1144-8. doi:10.1042/bst0361144


3 Goers L, Ainsworth C, Goey CH, Kontoravdi C, Freemont PS, Polizzi KM. Whole-cell Escherichia coli lactate biosensor for monitoring mammalian cell cultures during biopharmaceutical production. Biotechnol Bioeng. 2017;114(6):1290-1300. doi:10.1002/bit.26254