Difference between revisions of "Part:BBa K4255014"
 
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<partinfo>BBa_K4255014 short</partinfo>
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We divided the seven enzymes into two parts, 4CL, CHS and CHI catalyzed the production of naringenin one by one, and naringenin was further generated into anthocyanins in the presence of F3H, F3'5'H, DFR and ANS. The F3H, F3'5'H, DFR and ANS.
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<!-- sequences come from (哪四个物种). -->
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We constructed the last four enzymes on the pRSFDuet vector, in which F3H was fused to F3'5'H and DFR was fused to ANS.
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The function of the enzymes can be listed below:
 
F3H: catalyze flavanone into dihydrokaempferol
 
F3H: catalyze flavanone into dihydrokaempferol
 
F3’5’H: catalyze dihydrokaempferol into dihydromyricetin
 
F3’5’H: catalyze dihydrokaempferol into dihydromyricetin
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DFR: catalyze dihydromyricetin into delphinin colorless
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ANS: catalyze delphinin colorless into delphinidin
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4255013 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4255013 parameters</partinfo>
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Latest revision as of 07:36, 9 October 2022

This is a gene to code F3H, F3'5'H-DFR, ANS


We divided the seven enzymes into two parts, 4CL, CHS and CHI catalyzed the production of naringenin one by one, and naringenin was further generated into anthocyanins in the presence of F3H, F3'5'H, DFR and ANS. The F3H, F3'5'H, DFR and ANS. We constructed the last four enzymes on the pRSFDuet vector, in which F3H was fused to F3'5'H and DFR was fused to ANS. The function of the enzymes can be listed below: F3H: catalyze flavanone into dihydrokaempferol F3’5’H: catalyze dihydrokaempferol into dihydromyricetin DFR: catalyze dihydromyricetin into delphinin colorless ANS: catalyze delphinin colorless into delphinidin


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]