Difference between revisions of "Part:BBa K4192141"

 
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===Usage and Biology===
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===Usage===
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<p>As an indispensable part of the light control system, Yf1 affects the phosphorylation of FixL and then regulates the transcription of downstream genes by regulating the activity of the promoter.The YF1-containing light control system was introduced into the engineered bacteria to make them light-responsive and could be manipulated by light in a spatially and temporally accurate, reversible and non-harmful way.However,the N37C mutation of <i>yf1</i> has a better effect on regulation, that is, more target genes can be expressed under light conditions, and leakage expression can be minimized under light conditions.</p>
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 07:33, 9 October 2022


yf1(N37C)

The gene yf1 encodes a fusion kinase.The kinase yf1 ecxpressed controls gene expression in a light-dependent manner in vivo.The yf1 N37C can induce the expression of more downstream genes in the dark environment.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 544
    Illegal NgoMIV site found at 616
    Illegal NgoMIV site found at 706
    Illegal NgoMIV site found at 724
    Illegal AgeI site found at 258
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 157