Difference between revisions of "Part:BBa K4156113"

Revision as of 07:09, 9 October 2022

plac-mRFP

plac-mRFP is a biobrick that expresses mRFP controlled by the plac operon ( BBa_R0010 ). plac-mRFP functions as a red fluorescent signal to characterize the performance of the plac operon

Usage and Biology

In this biobrick, we used the plac operon to regulate downstream mRFP expression and eventually introduced the rrnB T1 and T7Te dual terminators. this design allowed the spatiotemporal intensity of plac manipulator-regulated gene expression to be measured by the red fluorescent signal. We were thus able to verify the specific response of the plac operon to lactose.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 864
Illegal AgeI site found at 976
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4156113 short</partinfo>
-To be refiend
+plac-mRFP is a biobrick that expresses mRFP controlled by the plac operon (<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_R0010"> BBa_R0010 </a></html>). plac-mRFP functions as a red fluorescent signal to characterize the performance of the plac operon
+<!-- Add more about the biology of this part here -->
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
-<!-- -->
+In this biobrick, we used the plac operon to regulate downstream mRFP expression and eventually introduced the rrnB T1 and T7Te dual terminators. this design allowed the spatiotemporal intensity of plac manipulator-regulated gene expression to be measured by the red fluorescent signal. We were thus able to verify the specific response of the plac operon to lactose.
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K4156113 SequenceAndFeatures</partinfo>