Difference between revisions of "Part:BBa K4137003:Design"
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YebF is chosen for the secretion pathway—SecB-SecA-SecYEG, OmpF. We chose to use the chi18h8 coding sequence as its produced chitinase has the ability to defunction Foc TR4 through lysing the fungus’ chitin. The 6xHis is used for protein quantification and is the most commonly used purification his-tag. | YebF is chosen for the secretion pathway—SecB-SecA-SecYEG, OmpF. We chose to use the chi18h8 coding sequence as its produced chitinase has the ability to defunction Foc TR4 through lysing the fungus’ chitin. The 6xHis is used for protein quantification and is the most commonly used purification his-tag. | ||
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| + | [[File:his-chitinase-gs-yebf.png|800px|thumb|center|Fig.Benchling Linear Map View of the BBa_K4137003 Basic Part.]] | ||
Latest revision as of 06:44, 9 October 2022
YebF is chosen for the secretion pathway—SecB-SecA-SecYEG, OmpF. We chose to use the chi18h8 coding sequence as its produced chitinase has the ability to defunction Foc TR4 through lysing the fungus’ chitin. The 6xHis is used for protein quantification and is the most commonly used purification his-tag.
