Difference between revisions of "Part:BBa K4156101:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
To be refined
 
  
 +
In the Llprd operon, we were the first to use a combination of the promoter hyper-spnak with RBS spoVG to initiate downstream sequences. After the lldR regulatory protein with the LldPRD promoter, we also introduced the RiboJ and RBS B0034 sequences to enhance transcriptional efficiency.
  
  
 
===Source===
 
===Source===
  
−
To be refined
+
Experimental constructed.
  
 
===References===
 
===References===
 +
 +
<i>
 +
Dual role of lldR in regulation of the lldPRD operon, involved in L-lactate metabolism in E. coli. Aguilera et al. J. Bacteriol. 2008, 190(8):2997
 +
</i>

Latest revision as of 06:21, 9 October 2022


pLldR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 688
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In the Llprd operon, we were the first to use a combination of the promoter hyper-spnak with RBS spoVG to initiate downstream sequences. After the lldR regulatory protein with the LldPRD promoter, we also introduced the RiboJ and RBS B0034 sequences to enhance transcriptional efficiency.


Source

Experimental constructed.

References

Dual role of lldR in regulation of the lldPRD operon, involved in L-lactate metabolism in E. coli. Aguilera et al. J. Bacteriol. 2008, 190(8):2997