Difference between revisions of "Part:BBa K4343095"

 
 
(2 intermediate revisions by the same user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K4343095 short</partinfo>
 
<partinfo>BBa_K4343095 short</partinfo>
  
EgElo9 encodes &#8710;-9 elongase from Euglena gracilis which functions in the &#916;-9 elongase/desaturase pathway and could convert C18:2 to C20:2.
+
EgElo9 encodes -9 elongase from Euglena gracilis which functions in the Δ-9 elongase/desaturase pathway and could convert C18:2 to C20:2.
 +
 
 +
====Characterization====
 +
 
 +
The EgElo9 gene encodes a protein of 258 amino acids. Heterologous expression of EgElo9 could catalyze linoleic acid (LA, C18:2) to eicosadienoic acid (EDA; C20: 2n-6).
 +
 
 +
====Reference====
 +
 
 +
1. Zhu, Q. 365, 307–316 (1999). et al. Metabolic engineering of an oleaginous yeast for the production of omega-3 fatty acids. in Single Cell Oils: Microbial and Algal Oils (eds. Cohen, Z.
 +
12. & Ratledge, C.) 51–73 (AOCS press, Urbana, 2010).
 +
 
 +
 
  
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
  
 
<!-- -->
 
<!-- -->

Latest revision as of 06:19, 9 October 2022


EgElo9

EgElo9 encodes ∆-9 elongase from Euglena gracilis which functions in the Δ-9 elongase/desaturase pathway and could convert C18:2 to C20:2.

Characterization

The EgElo9 gene encodes a protein of 258 amino acids. Heterologous expression of EgElo9 could catalyze linoleic acid (LA, C18:2) to eicosadienoic acid (EDA; C20: 2n-6).

Reference

1. Zhu, Q. 365, 307–316 (1999). et al. Metabolic engineering of an oleaginous yeast for the production of omega-3 fatty acids. in Single Cell Oils: Microbial and Algal Oils (eds. Cohen, Z. 12. & Ratledge, C.) 51–73 (AOCS press, Urbana, 2010).



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 354
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 794