Difference between revisions of "Part:BBa K4156101"

 
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<partinfo>BBa_K4156101 short</partinfo>
 
<partinfo>BBa_K4156101 short</partinfo>
  
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An Operon unit consisting of a collection of LldR-regulated promoters, and is capable of responding to lactate concentration.
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===Usage and Biology===
 
===Usage and Biology===
  
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Llprd operon is composed of the lldR regulatory protein (<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K1847001"> BBa_K1847001 </a></html>), the terminator B0012, and the LldPRD promoter（<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_M36021"> BBa_M36021 </a></html>）. At the LldPRD promoter, there are two operator sites O1 and O2 , which are able to bind to the lldR protein and block subsequent gene transcription. The presence of lactic acid molecules can unblock this blocking phenomenon. Thus, the Llprd operon can specifically respond to lactate and activate downstream genes. In our experiments, we used Llprd operon to adapt to the high lactate characteristics of the tumor cell microenvironment. Enabling our therapeutic strains to rapidly target to tumor cells and achieve targeted therapy.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4156101 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4156101 SequenceAndFeatures</partinfo>

Revision as of 06:16, 9 October 2022


pLldR

An Operon unit consisting of a collection of LldR-regulated promoters, and is capable of responding to lactate concentration.


Usage and Biology

Llprd operon is composed of the lldR regulatory protein ( BBa_K1847001 ), the terminator B0012, and the LldPRD promoter（ BBa_M36021 ）. At the LldPRD promoter, there are two operator sites O1 and O2 , which are able to bind to the lldR protein and block subsequent gene transcription. The presence of lactic acid molecules can unblock this blocking phenomenon. Thus, the Llprd operon can specifically respond to lactate and activate downstream genes. In our experiments, we used Llprd operon to adapt to the high lactate characteristics of the tumor cell microenvironment. Enabling our therapeutic strains to rapidly target to tumor cells and achieve targeted therapy.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 688
  • 1000
    COMPATIBLE WITH RFC[1000]