Difference between revisions of "Part:BBa K4137009:Design"
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T7 overexpression, RBS (AAGGAG) efficient protein expression, yebF export chitinase from periplasm to extracellular space via Omp pathway.
 
T7 overexpression, RBS (AAGGAG) efficient protein expression, yebF export chitinase from periplasm to extracellular space via Omp pathway.
  
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[[File:chi18h8-full.png|800px|thumb|center|Fig.1 Benchling Linear Map View of the BBa_K4137009 Composite Part.]]
 
  
 
===Source===
 
===Source===

Revision as of 06:00, 9 October 2022


T7+RBS+YebF-GS Linker-chi18h8-6xHis+B1006


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1523
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1036
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 583
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T7 overexpression, RBS (AAGGAG) efficient protein expression, yebF export chitinase from periplasm to extracellular space via Omp pathway.


Source

https://parts.igem.org/Part:BBa_K1659003

References