Difference between revisions of "Part:BBa K4343125"
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Derived from Y. lipolytica, GAP promotor is a specific DNA sequence upstream of the 5' end of a structural gene that can be specifically recognized and bound by RNAP. | Derived from Y. lipolytica, GAP promotor is a specific DNA sequence upstream of the 5' end of a structural gene that can be specifically recognized and bound by RNAP. | ||
+ | Some researchers have characterized multiple promoters by reporters, among which the GAP promoter facilitates the expression. | ||
+ | |||
+ | Reference: | ||
+ | Wong, L., Engel, J., Jin, E., Holdridge, B., & Xu, P. (2017). YaliBricks, a versatile genetic toolkit for streamlined and rapid pathway engineering in Yarrowia lipolytica. Metabolic engineering communications, 5, 68-77. | ||
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Latest revision as of 05:56, 9 October 2022
GAP
Derived from Y. lipolytica, GAP promotor is a specific DNA sequence upstream of the 5' end of a structural gene that can be specifically recognized and bound by RNAP. Some researchers have characterized multiple promoters by reporters, among which the GAP promoter facilitates the expression.
Reference: Wong, L., Engel, J., Jin, E., Holdridge, B., & Xu, P. (2017). YaliBricks, a versatile genetic toolkit for streamlined and rapid pathway engineering in Yarrowia lipolytica. Metabolic engineering communications, 5, 68-77.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 489
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 564