Difference between revisions of "Part:BBa K4137008"

Revision as of 05:37, 9 October 2022

mleR expressing construct

This construct produces and purifies mleR, the malate-induced regulator that binds to p_mleS promoter for the production of ccdA antitoxin.

Fig.1 Malate-binding transcriptional activator + 6x His-Tag complete construct.

Construct Designs

We attached a 6x His-Tag downstream of the mleR sequence for purification purposes. A T7 and RBS are attached upstream to the side of the open reading frame. The terminator BBa_B1006 is attached downstream of the sequence.

Characterization

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 49
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4137008 short</partinfo>
-This construct contains a T7 promoter, a RBS, a mleR coding complex, a mleR purification tag—6xHis, and a terminator B1006. This construct produce and purifies mleR, the regulator of CcdA, which produces when malate acid is presented.
+This construct produces and purifies mleR, the malate-induced regulator that binds to p_mleS promoter for the production of ccdA antitoxin.
 [[File:t7-mler-his.png|800px|thumb|center|Fig.1 Malate-binding transcriptional activator + 6x His-Tag complete construct.]]