Difference between revisions of "Part:BBa K4137006"
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The mleR regulatory site which comes from p_mles binds with the RBS and CcdA, the antitoxin that will bind with CcdB when induced by malate acid and detoxifies it, the purification tag 6xHis, and the terminator B1006 (BBa_B1006). This sequence codes for CcdA, the antitoxin which assures chi18h8’s production around the plant roots. | The mleR regulatory site which comes from p_mles binds with the RBS and CcdA, the antitoxin that will bind with CcdB when induced by malate acid and detoxifies it, the purification tag 6xHis, and the terminator B1006 (BBa_B1006). This sequence codes for CcdA, the antitoxin which assures chi18h8’s production around the plant roots. | ||
| − | < | + | <-- Add more about the biology of this part here |
| − | === | + | |
| + | ===Construct Designs=== | ||
| + | |||
| + | We attached a 6x His-Tag downstream of the ccdA sequence for purification purposes. A p_mleS promoter, which induces protein production when malate is present, is attached upstream to the side of the open reading frame + RBS. The terminator BBa_B1006 is attached downstream of the sequence. | ||
| + | |||
| + | ===Characterization=== | ||
| − | |||
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Revision as of 03:59, 9 October 2022
CcdA expressing construct
The mleR regulatory site which comes from p_mles binds with the RBS and CcdA, the antitoxin that will bind with CcdB when induced by malate acid and detoxifies it, the purification tag 6xHis, and the terminator B1006 (BBa_B1006). This sequence codes for CcdA, the antitoxin which assures chi18h8’s production around the plant roots.
<-- Add more about the biology of this part here
Construct Designs
We attached a 6x His-Tag downstream of the ccdA sequence for purification purposes. A p_mleS promoter, which induces protein production when malate is present, is attached upstream to the side of the open reading frame + RBS. The terminator BBa_B1006 is attached downstream of the sequence.
Characterization
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]