Difference between revisions of "Part:BBa K4137006"
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===Usage and Biology===
 
===Usage and Biology===
  
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[[File:pmles-ccda-his.png.png|800px|thumb|center|Fig.1 Malate-inducible ccdA full construct.]]
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Revision as of 03:27, 9 October 2022


CcdA expressing construct

The mleR regulatory site which comes from p_mles binds with the RBS and CcdA, the antitoxin that will bind with CcdB when induced by malate acid and detoxifies it, the purification tag 6xHis, and the terminator B1006 (BBa_B1006). This sequence codes for CcdA, the antitoxin which assures chi18h8’s production around the plant roots.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]