Difference between revisions of "Part:BBa K4477012:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Amino acid sequences reverse transcribed and codon optimized for expression in E. coli B (the parent strain of SHuffle).
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Amino acid sequences were reverse transcribed and codon optimized for expression in E. coli B (the parent strain of SHuffle). In addition, further codon optimization was used to remove illegal restriction sites from the construct.
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===Source===
 
===Source===

Revision as of 02:48, 9 October 2022


IK17 (anti-oxLDL) scFv - complete expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 576
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amino acid sequences were reverse transcribed and codon optimized for expression in E. coli B (the parent strain of SHuffle). In addition, further codon optimization was used to remove illegal restriction sites from the construct.

Source

1. https://www.jacc.org/doi/full/10.1016/j.jacc.2011.07.017 2. https://pubmed.ncbi.nlm.nih.gov/14644097/

References