Difference between revisions of "Part:BBa K143057"

Revision as of 02:37, 9 October 2022

Promoter xyl and RBS spoVG for B subtilis

Inducible promoter xyl(BBa_K143014) coupled to the strong Ribosome Binding Site spoVG(BBa_K143021) from B. subtilis.

Pxyl-spoVG can be used to take an input of xylose and give a Ribosomes per second (RiPS) output generator.

Xylose does not directly induce the expression of the promoter xyl, but requires the transcriptional regulator XylR, (BBa_K143036). This means that XylR must be constitutively expressed in B.subtilis in order to use the promoter hyper-spank as an inducible promoter. XylR is naturally expressed by B. subtilis but should be upregulated to increase efficiency.

To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

1 https://parts.igem.org/Assembly:RBS-CDS_issues

</biblio>

Contribution:iGEM22_WHU-China

Figure. 1 Expression of Pet8p in E. coli and transportation efficiency measurement in liposome

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 #1 https://parts.igem.org/Assembly:RBS-CDS_issues
 </biblio>
+==Contribution:iGEM22_WHU-China==
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+        <div class="col-lg" style="margin:auto;text-align:center;">
+                 <img style="margin:20px auto 5px auto;" src="https://static.igem.wiki/teams/4144/wiki/same-part/bba-k4144004/picture1.png" width="60%">
+                 <p style="color:Gray; padding:0px 30px 10px;">Figure. 1 Expression of  Pet8p in E. coli and transportation efficiency measurement in liposome</p>
+         </div>
+</html>
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