Difference between revisions of "Part:BBa K4147001:Design"
 
(2 intermediate revisions by one other user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
It is widely use in eukaryotic cells. In addition, as ist is an antimicrobial peptide is desired to express it under medium strenght promoter, in order to mantain the concentration of a toxic target protein just below a host strain's tolerance. The PelB sequence allows the peptide to be sent to a periplasmic space for easier purification and an histidine tag for IMAC purification.
+
It is widely use in eukaryotic cells. In addition, as it is an antimicrobial peptide is desired to express it under medium strength promoter, in order to maintain the concentration of a toxic target protein just below a host strain's tolerance. The PelB sequence allows the peptide to be sent to a periplasmic space for easier purification and a histidine tag for IMAC purification.
−
 
+
  
  
 
===Source===
 
===Source===
  
−
This sequence was obtained from the GenBank (MW048779.1) as a synthetic construct fot th production of a recombinant Dermaseptin peptide in <i>Nicotiana tabacum</i> hairy roots with enhanced antimicrobial activity.
+
This sequence was obtained from the GenBank (MW048779.1) as a synthetic construct for the production of a recombinant Dermaseptin peptide in <i>Nicotiana tabacum</i> hairy roots with enhanced antimicrobial activity.
−
 
+
−
Shams, M.V., Nazarian-Firouzabadi, F., Ismaili, A. et al. Production of a Recombinant Dermaseptin Peptide in Nicotiana tabacum Hairy Roots with Enhanced Antimicrobial Activity. Mol Biotechnol 61, 241–252 (2019). https://doi.org/10.1007/s12033-019-00153-x 
+
  
 
===References===
 
===References===
 
Shams, M.V., Nazarian-Firouzabadi, F., Ismaili, A. et al. Production of a Recombinant Dermaseptin Peptide in Nicotiana tabacum Hairy Roots with Enhanced Antimicrobial Activity. Mol Biotechnol 61, 241–252 (2019). https://doi.org/10.1007/s12033-019-00153-x
 
Shams, M.V., Nazarian-Firouzabadi, F., Ismaili, A. et al. Production of a Recombinant Dermaseptin Peptide in Nicotiana tabacum Hairy Roots with Enhanced Antimicrobial Activity. Mol Biotechnol 61, 241–252 (2019). https://doi.org/10.1007/s12033-019-00153-x

Latest revision as of 02:34, 9 October 2022


PelB-(CBD)2-DrsB1: A dermaseptin antimicrobial peptide from Phyllomedusa bicolor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 165
    Illegal AgeI site found at 444
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is widely use in eukaryotic cells. In addition, as it is an antimicrobial peptide is desired to express it under medium strength promoter, in order to maintain the concentration of a toxic target protein just below a host strain's tolerance. The PelB sequence allows the peptide to be sent to a periplasmic space for easier purification and a histidine tag for IMAC purification.


Source

This sequence was obtained from the GenBank (MW048779.1) as a synthetic construct for the production of a recombinant Dermaseptin peptide in Nicotiana tabacum hairy roots with enhanced antimicrobial activity.

References

Shams, M.V., Nazarian-Firouzabadi, F., Ismaili, A. et al. Production of a Recombinant Dermaseptin Peptide in Nicotiana tabacum Hairy Roots with Enhanced Antimicrobial Activity. Mol Biotechnol 61, 241–252 (2019). https://doi.org/10.1007/s12033-019-00153-x