Difference between revisions of "Part:BBa K4147002:Design"

 
 
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4147002 SequenceAndFeatures</partinfo>
  
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===DESIGN NOTES===
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It is widely use in eukaryotic cells. In addition, as ist is an antimicrobial peptide is desired to express it under medium strenght promoter, in order to mantain the concentration of a toxic target protein just below a host strain's tolerance. The PelB sequence allows the peptide to be sent to a periplasmic space for easier purification and an histidine tag for IMAC purification.
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===SOURCE===
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This sequence was obtained from the GenBank (MW048779.1) as a synthetic construct fot th production of a recombinant Dermaseptin peptide in <i>Nicotiana tabacum</i> hairy roots with enhanced antimicrobial activity.

Latest revision as of 02:32, 9 October 2022

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 392
    Illegal AgeI site found at 671
  • 1000
    COMPATIBLE WITH RFC[1000]

DESIGN NOTES

It is widely use in eukaryotic cells. In addition, as ist is an antimicrobial peptide is desired to express it under medium strenght promoter, in order to mantain the concentration of a toxic target protein just below a host strain's tolerance. The PelB sequence allows the peptide to be sent to a periplasmic space for easier purification and an histidine tag for IMAC purification.

SOURCE

This sequence was obtained from the GenBank (MW048779.1) as a synthetic construct fot th production of a recombinant Dermaseptin peptide in Nicotiana tabacum hairy roots with enhanced antimicrobial activity.