Difference between revisions of "Part:BBa K4147003"
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<partinfo>BBa_K4147003 short</partinfo>
 
<partinfo>BBa_K4147003 short</partinfo>
  
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This part contains the linear construct for pcOSM (plant osmotin from Piper colubrinum) with an included LacI promoter (1118 pb in total). Additionally, it contains an RBS under the part name BBa_B0032, as well as a 6X-His tag at the end of the osmotin CDS for later protein purification. Finally, it has a double terminator (BBa_B0015) to ensure correct termination. As a linear construct, it contains the RFC10 prefix and suffix, making the part compatible with other iGEM parts.  
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This part contains the linear construct for pcOSM (plant osmotin from Piper colubrinum) with an included LacI promoter. Additionally, it contains an RBS under the part name BBa_B0032, as well as a 6X-His tag at the end of the osmotin CDS for later protein purification. Finally, it has a double terminator (BBa_B0015) to ensure correct termination. As a linear construct, it contains the RFC10 prefix and suffix, making the part compatible with other iGEM parts.  
  
  

Revision as of 02:29, 9 October 2022


PcOSM Construct with LacI regulated promoter

This part contains the linear construct for pcOSM (plant osmotin from Piper colubrinum) with an included LacI promoter. Additionally, it contains an RBS under the part name BBa_B0032, as well as a 6X-His tag at the end of the osmotin CDS for later protein purification. Finally, it has a double terminator (BBa_B0015) to ensure correct termination. As a linear construct, it contains the RFC10 prefix and suffix, making the part compatible with other iGEM parts.


Usage and Biology

This osmotin is a plant defense protein with a molecular weight of ~24 kDa. PcOSM belongs to pathogenesis related-5 (PR-5) family, which is accumulated in response to both biotic and abiotic stresses [1]. This osmotin has exhibited significant inhibitory activity against the oomycete pathogen Phytophthora capsici. This protein is able to inhibit fungal growth through inhibition of hyphal growth, spore germination, spore lysis, and reduction in viability of spore [2]. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 806
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 498
  • 1000
    COMPATIBLE WITH RFC[1000]


REFERENCES

[1] Geetha, R. G., Krishnankutty Nair Chandrika, S., Saraswathy, G. G., Nair Sivakumari, A., & Sakuntala, M. (2021). ROS Dependent Antifungal and Anticancer Modulations of Piper colubrinum Osmotin. Molecules, 26(8), 2239. doi:10.3390/molecules26082239

[2] Chowdhury, S., Basu, A., & Kundu, S. (2015). Cloning, Characterization, and Bacterial Over-Expression of an Osmotin-like Protein Gene from Solanum nigrum L. with Antifungal Activity Against Three Necrotrophic Fungi. Molecular Biotechnology, 57(4), 371–381. doi:10.1007/s12033-014-9831-4