Difference between revisions of "Part:BBa K4345007"
 
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NarX is a part of NarX-NarL two component nitrate sensing system in Escherichia coli. They are responsible for the expression of anaerobic respiratory genes. NarX is a histidine kinases that independently detect the presence of nitrate an transmit the signal to NarL. After autophosphorylation and thus dimerization of NarX or NarQ, NarL is phosphorylated. This enables the activated NarL to bind DNA and induce expression of specific genes. Both NarX and narQ contain two conserved histidine residues that correspond to the autophosphorylation sites of other, homologous, sensor-transmitter proteins (Cavicchioli et al., 1995). Cheung & Hendrickson (2009) elucidated the structure of the histidine kinase in the apo- and holo-state to be a four-helix bundle.
 
NarX is a part of NarX-NarL two component nitrate sensing system in Escherichia coli. They are responsible for the expression of anaerobic respiratory genes. NarX is a histidine kinases that independently detect the presence of nitrate an transmit the signal to NarL. After autophosphorylation and thus dimerization of NarX or NarQ, NarL is phosphorylated. This enables the activated NarL to bind DNA and induce expression of specific genes. Both NarX and narQ contain two conserved histidine residues that correspond to the autophosphorylation sites of other, homologous, sensor-transmitter proteins (Cavicchioli et al., 1995). Cheung & Hendrickson (2009) elucidated the structure of the histidine kinase in the apo- and holo-state to be a four-helix bundle.
  
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===Sequence and Features===
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<partinfo>BBa_K4345007 SequenceAndFeatures</partinfo>
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===Usage and Biology===
 
===Usage and Biology===
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This particular narX protein was derived from ''E. coli''.
  
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K4345007 SequenceAndFeatures</partinfo>
 
  
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[[Image:NarX nitrate Cheung&amp;Hendrickson2009.jpeg|500px]]
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Image obtained from Cheung & Hendrickson, 2009
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===References===
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Cavicchioli, R., Schro¨der, I., Schro¨der, S., Constanti, M., & Gunsalus, R. P. (1995). The NarX and NarQ Sensor-Transmitter Proteins of Escherichia coli Each Require Two Conserved Histidines for Nitrate-Dependent Signal Transduction to NarL. JOURNAL OF BACTERIOLOGY, 177(9), 2416–2424.
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Cheung, J., & Hendrickson, W. A. (2009). Structural Analysis of Ligand Stimulation of the Histidine Kinase NarX. Structure, 17(2), 190–201. https://doi.org/10.1016/J.STR.2008.12.013
  
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narX sensor histidine kinase NarX [ Escherichia coli str. K-12 substr. MG1655 ]. (2022, September 22). National Library of Medicine - National Center for Biotechnology Information. https://www.ncbi.nlm.nih.gov/gene/945788
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===Functional Parameters===
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<partinfo>BBa_K4345007 parameters</partinfo>
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Latest revision as of 22:53, 8 October 2022


NarX

NarX is a part of NarX-NarL two component nitrate sensing system in Escherichia coli. They are responsible for the expression of anaerobic respiratory genes. NarX is a histidine kinases that independently detect the presence of nitrate an transmit the signal to NarL. After autophosphorylation and thus dimerization of NarX or NarQ, NarL is phosphorylated. This enables the activated NarL to bind DNA and induce expression of specific genes. Both NarX and narQ contain two conserved histidine residues that correspond to the autophosphorylation sites of other, homologous, sensor-transmitter proteins (Cavicchioli et al., 1995). Cheung & Hendrickson (2009) elucidated the structure of the histidine kinase in the apo- and holo-state to be a four-helix bundle.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 659
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 659
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 260
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 659
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 659
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

This particular narX protein was derived from E. coli.


NarX nitrate Cheung&Hendrickson2009.jpeg

Image obtained from Cheung & Hendrickson, 2009

References

Cavicchioli, R., Schro¨der, I., Schro¨der, S., Constanti, M., & Gunsalus, R. P. (1995). The NarX and NarQ Sensor-Transmitter Proteins of Escherichia coli Each Require Two Conserved Histidines for Nitrate-Dependent Signal Transduction to NarL. JOURNAL OF BACTERIOLOGY, 177(9), 2416–2424.

Cheung, J., & Hendrickson, W. A. (2009). Structural Analysis of Ligand Stimulation of the Histidine Kinase NarX. Structure, 17(2), 190–201. https://doi.org/10.1016/J.STR.2008.12.013

narX sensor histidine kinase NarX [ Escherichia coli str. K-12 substr. MG1655 ]. (2022, September 22). National Library of Medicine - National Center for Biotechnology Information. https://www.ncbi.nlm.nih.gov/gene/945788