Difference between revisions of "Part:BBa K4268004:Experience"

Latest revision as of 18:22, 8 October 2022

The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5α cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.

Figure 1: Colony PCR of four colonies (clones) suspected of containing the Tail Tubular Protein A insert. The insert length is 615bp and the predicted size of the PCR product when using VF/VR primers is 920bp.

The gel indicates that all four colonies are likely to contain the correct insert and thus, the Tail Tubular Protein A was successfully cloned into a Level 0 Golden Gate Assembly basic part.

Applications of BBa_K4268004

User Reviews

UNIQ8258c5ae20d39e02-partinfo-00000000-QINU UNIQ8258c5ae20d39e02-partinfo-00000001-QINU

@@ Line 4: / Line 4: @@
-[[File: T-suny-oneonta-t7-tail tubular protein A colony PCR figure.png|500px|thumb|center|Figure 1: Colony PCR of five colonies (clones) suspected of coating the insert Head-tail connector insert. The insert length is 1573bp and the predicted size of the PCR product when using VF/VR primers is 1739 bp.]]
+[[File: T-suny-oneonta-t7-tail tubular protein A colony PCR figure.png|500px|thumb|center|Figure 1: Colony PCR of four colonies (clones) suspected of containing the Tail Tubular Protein A insert. The insert length is 615bp and the predicted size of the PCR product when using VF/VR primers is 920bp.]]
-The gel indicates that all five colonies are likely to contain the correct insert and thus, the Head-Tail Connector was successfully cloned into a Level O Golden Gate Assembly basic part.
+The gel indicates that all four colonies are likely to contain the correct insert and thus, the Tail Tubular Protein A was successfully cloned into a Level 0 Golden Gate Assembly basic part.
 ===Applications of BBa_K4268004===