Difference between revisions of "Part:BBa K4268001:Experience"

 
 
(7 intermediate revisions by the same user not shown)
Line 1: Line 1:
  
 
__NOTOC__
 
__NOTOC__
−
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
+
The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5&alpha; cells.  After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.
−
how you used this part and how it worked out.
+
 
 +
 
 +
[[File: T-suny-oneonta-t7-capsid assembly protein colony PCR figure.png|500px|thumb|center|Figure 1: Colony PCR of five colonies (clones) suspected of containing the Capsid Assembly Protein insert. The insert length is 768bp and the predicted size of the PCR product when using VF/VR primers is 1073 bp.]]
 +
 
 +
The gel indicates that colonies 1 and 2 are likely to contain the correct insert and thus, the Capsid Assembly Protein was successfully cloned into a Level 0 Golden Gate Assembly basic part.
 +
 
  
 
===Applications of BBa_K4268001===
 
===Applications of BBa_K4268001===
Line 15: Line 20:
 
<I>Username</I>
 
<I>Username</I>
 
|width='60%' valign='top'|
 
|width='60%' valign='top'|
−
Enter the review inofrmation here.
+
Enter the review information here.
 
|};
 
|};
 
<!-- End of the user review template -->
 
<!-- End of the user review template -->
 
<!-- DON'T DELETE --><partinfo>BBa_K4268001 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K4268001 EndReviews</partinfo>

Latest revision as of 18:21, 8 October 2022


The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5α cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.


Figure 1: Colony PCR of five colonies (clones) suspected of containing the Capsid Assembly Protein insert. The insert length is 768bp and the predicted size of the PCR product when using VF/VR primers is 1073 bp.

The gel indicates that colonies 1 and 2 are likely to contain the correct insert and thus, the Capsid Assembly Protein was successfully cloned into a Level 0 Golden Gate Assembly basic part.


Applications of BBa_K4268001

User Reviews

UNIQcecc801cd58246af-partinfo-00000000-QINU UNIQcecc801cd58246af-partinfo-00000001-QINU