Difference between revisions of "Part:BBa K4268002:Experience"

 
 
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The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5&alpha; cells.  After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.
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how you used this part and how it worked out.
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[[File: T-suny-oneonta-t7-capsid protein colony PCR figure.png|500px|thumb|center|Figure 1: Colony PCR of five colonies (clones) suspected of containing the Capsid Protein insert. The insert length is 720bp and the predicted size of the PCR product when using VF/VR primers is 1025 bp.]]
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The gel indicates that all five colonies are likely to contain the correct insert and thus, the Capsid Protein was successfully cloned into a Level 0 Golden Gate Assembly basic part.
  
 
===Applications of BBa_K4268002===
 
===Applications of BBa_K4268002===
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Latest revision as of 18:20, 8 October 2022


The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5α cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.


Figure 1: Colony PCR of five colonies (clones) suspected of containing the Capsid Protein insert. The insert length is 720bp and the predicted size of the PCR product when using VF/VR primers is 1025 bp.

The gel indicates that all five colonies are likely to contain the correct insert and thus, the Capsid Protein was successfully cloned into a Level 0 Golden Gate Assembly basic part.

Applications of BBa_K4268002

User Reviews

UNIQ144c69d71923436c-partinfo-00000000-QINU UNIQ144c69d71923436c-partinfo-00000001-QINU