Difference between revisions of "Part:BBa K4268010:Experience"
Jazmine124 (Talk | contribs) |
|||
| (One intermediate revision by the same user not shown) | |||
| Line 1: | Line 1: | ||
__NOTOC__ | __NOTOC__ | ||
| − | + | ||
| − | + | Level 0 parts BBa_R0085, BBa_B0034, BBa_K4268002 (Capsid Protein), and BBa_B0015 were cloned into the level 1 Golden Gate vector, PSB1K02. The ligation was then transformed into DH5α cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair. | |
| + | |||
| + | [[File: T-suny-oneonta-t7-Level 1 capsid protein colony PCR figure.png|500px|thumb|center|Figure 1: Colony PCR of five colonies (clones) suspected of containing the Capsid Protein insert. The insert length is 906bp (including the fusion sites) and the predicted size of the PCR product when using VF/VR primers is 1211 bp.]] | ||
| + | |||
| + | The gel indicates that all five colonies are likely to contain the correct insert and thus, the Level 1 Capsid Protein Transcriptional Unit was successfully cloned. | ||
| + | |||
===Applications of BBa_K4268010=== | ===Applications of BBa_K4268010=== | ||
Latest revision as of 18:19, 8 October 2022
Level 0 parts BBa_R0085, BBa_B0034, BBa_K4268002 (Capsid Protein), and BBa_B0015 were cloned into the level 1 Golden Gate vector, PSB1K02. The ligation was then transformed into DH5α cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.
The gel indicates that all five colonies are likely to contain the correct insert and thus, the Level 1 Capsid Protein Transcriptional Unit was successfully cloned.
Applications of BBa_K4268010
User Reviews
UNIQ2bff3a8f5e1d4065-partinfo-00000000-QINU UNIQ2bff3a8f5e1d4065-partinfo-00000001-QINU