Difference between revisions of "Part:BBa K4339003"
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<partinfo>BBa_K4339003 short</partinfo> | <partinfo>BBa_K4339003 short</partinfo> | ||
| − | A major ampullate spidroin 1 (MaSp1) based protein consisting of the Great Bay 2019 part <partinfo>BBa_K3264003</partinfo> fused to | + | A major ampullate spidroin 1 (MaSp1) based protein consisting of the Great Bay 2019 part <partinfo>BBa_K3264003</partinfo> fused to <partinfo>BBa_K3264002</partinfo>. Due to the highly repetitive nature of spidroin proteins, the DNA coding sequences are also highly repetitive. Unfortunately we were unable to submit the original Great Bay sequence for synthesis, and therefore K3264003 had to be modified to allow for synthesis. |
Revision as of 16:24, 8 October 2022
MaSp1_K3264003mod_K3264002
A major ampullate spidroin 1 (MaSp1) based protein consisting of the Great Bay 2019 part BBa_K3264003 fused to BBa_K3264002. Due to the highly repetitive nature of spidroin proteins, the DNA coding sequences are also highly repetitive. Unfortunately we were unable to submit the original Great Bay sequence for synthesis, and therefore K3264003 had to be modified to allow for synthesis.
Usage and Biology
Sequence and Features
The sequence originates from the African nursery web spider Euprosthenops australis (Stark et al. 2007).
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Stark, M. et al. (2007) ‘Macroscopic fibers self-assembled from recombinant miniature spider silk proteins’, Biomacromolecules, 8(5), pp. 1695–1701. Available at: https://doi.org/10.1021/bm070049y.