Difference between revisions of "Part:BBa K4242004"
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<partinfo>BBa_K4242004 short</partinfo>
 
<partinfo>BBa_K4242004 short</partinfo>
  
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This part sequence originated from Escherichia coli encodes YedQ protein. YedQ is diguanylate cyclase.
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This part sequence originated from <i>Escherichia coli</i> encodes YedQ protein. YedQ is diguanylate cyclase.
  
 
===Usage and Biology===
 
===Usage and Biology===
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YedQ can work with the phosphodiesterase (YhjH) modulated the in vivo c-di-GMP levels. The expression of the yedQ gene increased the intracellular c-di-GMP concentration and resulted in the enhanced secretion of EPS. So, The protein YedQ can promote bacterial self-aggregation and adhesion onto negatively charged surfaces.
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YedQ can work with the phosphodiesterase (YhjH) modulated the in vivo c-di-GMP levels. The expression of the yedQ gene increased the intracellular c-di-GMP concentration and resulted in the enhanced secretion of EPS[1]. So, The protein YedQ can promote bacterial self-aggregation and adhesion onto negatively charged surfaces. In <i>E. coli</i>, extracellular DNA (eDNA) was increased as expected for the deletions of yedQ. As a result of the significantly enhanced motility, but contrary to current models of decreased biofilm formation with decreased diguanylate cyclase activity, early biofilm formation increased dramatically for the deletions of yedQ[2].
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<h3><center>The quantification of intracellular c-di-GMP</center></h3>
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<div class = "center">
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<center>https://static.igem.org/mediawiki/parts/4/48/YedQ.png</center>
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</div>
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<center><b>Fig. 1.</b> The quantification of intracellular c-di-GMP in C. testosteroni WDL7/pYedQ2, the wild-type strain and WDL7/YhjH. nd, not determined (a). Crystal violet staining of WDL7/pYedQ2, WDL7 and WDL7/YhjH strains for quantifying relative biofilm biomass (b). * indicates significantly difference when compared with the WDL7 wild type strain, * p < 0.05, *** p < 0.001[1].</center>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4242004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4242004 SequenceAndFeatures</partinfo>
  
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===References===
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[1] Yang S, Wu Y, Qu C, et al. Quantitative analysis of the surficial and adhesion properties of the Gram-negative bacterial species Comamonas testosteroni modulated by c-di-GMP[J]. Colloids Surf B Biointerfaces, 2021,198:111497.
  
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[2] Sanchez-Torres V, Hu H, Wood T K. GGDEF proteins YeaI, YedQ, and YfiN reduce early biofilm formation and swimming motility in Escherichia coli[J]. Appl Microbiol Biotechnol, 2011,90(2):651-658.
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===Functional Parameters===
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<partinfo>BBa_K4242004 parameters</partinfo>
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Latest revision as of 15:50, 8 October 2022


yedQ

This part sequence originated from Escherichia coli encodes YedQ protein. YedQ is diguanylate cyclase.

Usage and Biology

YedQ can work with the phosphodiesterase (YhjH) modulated the in vivo c-di-GMP levels. The expression of the yedQ gene increased the intracellular c-di-GMP concentration and resulted in the enhanced secretion of EPS[1]. So, The protein YedQ can promote bacterial self-aggregation and adhesion onto negatively charged surfaces. In E. coli, extracellular DNA (eDNA) was increased as expected for the deletions of yedQ. As a result of the significantly enhanced motility, but contrary to current models of decreased biofilm formation with decreased diguanylate cyclase activity, early biofilm formation increased dramatically for the deletions of yedQ[2].

The quantification of intracellular c-di-GMP

YedQ.png
Fig. 1. The quantification of intracellular c-di-GMP in C. testosteroni WDL7/pYedQ2, the wild-type strain and WDL7/YhjH. nd, not determined (a). Crystal violet staining of WDL7/pYedQ2, WDL7 and WDL7/YhjH strains for quantifying relative biofilm biomass (b). * indicates significantly difference when compared with the WDL7 wild type strain, * p < 0.05, *** p < 0.001[1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1591
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1] Yang S, Wu Y, Qu C, et al. Quantitative analysis of the surficial and adhesion properties of the Gram-negative bacterial species Comamonas testosteroni modulated by c-di-GMP[J]. Colloids Surf B Biointerfaces, 2021,198:111497.

[2] Sanchez-Torres V, Hu H, Wood T K. GGDEF proteins YeaI, YedQ, and YfiN reduce early biofilm formation and swimming motility in Escherichia coli[J]. Appl Microbiol Biotechnol, 2011,90(2):651-658.