Difference between revisions of "Part:BBa K4339000:Design"

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===Design Notes===
 
===Design Notes===
−
The sequence was modified to remove forbidden restriction sites to allow for type IIS cloning.
+
The sequence was modified to remove forbidden restriction sites to allow for Type IIS cloning.
  
 
===Source===
 
===Source===
  
−
The CycA sequence originates in a K-12 strain of <i>E. coli</i>.
+
The CycA sequence originates from <i>E. coli</i> MG1655 K-12.
  
 
===References===
 
===References===

Latest revision as of 15:13, 8 October 2022


Non-polar amino acid transporter (CycA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 340
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was modified to remove forbidden restriction sites to allow for Type IIS cloning.

Source

The CycA sequence originates from E. coli MG1655 K-12.

References