Difference between revisions of "Part:BBa K4345020:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | Our team used the RNA thermometer as a part of a killswitch. The sequence was implemented before an antitoxin (ccdB) to regulate its translation based on temperature. It is possible to implement a part of the sequence of the gene that will be translated within the sequence that forms secondary hairpin structure. By doing this, leaky expression will be reduced. Because of the typical short sequence of this thermometer and the position of the startcodon, our team decided not to. The position of the startcodon can be found in the provided figure. | |
− | + | ||
===Source=== | ===Source=== | ||
− | + | Implemented in the mRNA structure of a cyanobacterial (''Synechocystis sp.'') heatshock gene which is important for stress tolerance. | |
===References=== | ===References=== | ||
+ | Kortmann, J., Sczodrok, S., Rinnenthal, J., Schwalbe, H., & Narberhaus, F. (2010, December 3). Translation on demand by a simple RNA-based thermosensor. Nucleic Acids Research, 39(7), 2855–2868. https://doi.org/10.1093/nar/gkq1252 |
Latest revision as of 15:04, 8 October 2022
5' UTR of hsp17 with ccdA fused to sfGFP
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1149
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 569
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Our team used the RNA thermometer as a part of a killswitch. The sequence was implemented before an antitoxin (ccdB) to regulate its translation based on temperature. It is possible to implement a part of the sequence of the gene that will be translated within the sequence that forms secondary hairpin structure. By doing this, leaky expression will be reduced. Because of the typical short sequence of this thermometer and the position of the startcodon, our team decided not to. The position of the startcodon can be found in the provided figure.
Source
Implemented in the mRNA structure of a cyanobacterial (Synechocystis sp.) heatshock gene which is important for stress tolerance.
References
Kortmann, J., Sczodrok, S., Rinnenthal, J., Schwalbe, H., & Narberhaus, F. (2010, December 3). Translation on demand by a simple RNA-based thermosensor. Nucleic Acids Research, 39(7), 2855–2868. https://doi.org/10.1093/nar/gkq1252