Difference between revisions of "Part:BBa K4339000:Design"

(Source)
(Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
The sequence was modified to remove forbidden restriction sites to allow for type IIS cloning.
+
The sequence was modified to remove forbidden restriction sites to allow for Type IIS cloning.
  
 
===Source===
 
===Source===

Revision as of 14:59, 8 October 2022


Non-polar amino acid transporter (CycA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 340
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was modified to remove forbidden restriction sites to allow for Type IIS cloning.

Source

The CycA sequence originates in a K-12 strain of E. coli.

References