Difference between revisions of "Part:BBa K4325010"
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<p>Our goal is to reduce the leakage level of pDawn system by removing the degradation tag LVA in the original repressor cI as increasing the concentration of cI could enhance the repression of PR promoter.</p> | <p>Our goal is to reduce the leakage level of pDawn system by removing the degradation tag LVA in the original repressor cI as increasing the concentration of cI could enhance the repression of PR promoter.</p> | ||
[[ File:Improvement3.jpeg|800px|thumb|center||Figure 1. Sequence alignment of pDawn(cI-LVA) before and after removing LVA tag]] | [[ File:Improvement3.jpeg|800px|thumb|center||Figure 1. Sequence alignment of pDawn(cI-LVA) before and after removing LVA tag]] | ||
| + | <h3>Usage</h3> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 14:40, 8 October 2022
pDawn without LVA tag
Description
Our reduce the leakage level of pDawn system by removing the degradation tag LVA in the original repressor cI .
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2171
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 63
Illegal NgoMIV site found at 195
Illegal NgoMIV site found at 289
Illegal NgoMIV site found at 582
Illegal NgoMIV site found at 1076
Illegal NgoMIV site found at 1094
Illegal NgoMIV site found at 1184
Illegal AgeI site found at 414
Illegal AgeI site found at 1542 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1643
Illegal BsaI.rc site found at 525
2022 SZPT-China
Biology
Our goal is to reduce the leakage level of pDawn system by removing the degradation tag LVA in the original repressor cI as increasing the concentration of cI could enhance the repression of PR promoter.
