Difference between revisions of "Part:BBa K228866:Design"

 
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<partinfo>BBa_K228866 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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part registery 2009 distribution & PKU igem 2009 team
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when cut this part for insert and ligate it into a vetor, the insert and vector may have the similar sizes which may disturb the ligation.
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===Source===
 
===Source===
  
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when cut this part for insert and ligate it into a vetor, the insert and vector may have the similar sizes which may disturb the ligation.
+
part registery 2009 distribution & PKU 2009 igem team
  
 
===References===
 
===References===

Latest revision as of 10:15, 22 September 2009

AND Gate pBad-SupD pSal-PhiR73 delta(amber mutation)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1274
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2007
    Illegal BamHI site found at 1214
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1839
    Illegal AgeI site found at 1320
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1323


Design Notes

when cut this part for insert and ligate it into a vetor, the insert and vector may have the similar sizes which may disturb the ligation.

Source

part registery 2009 distribution & PKU 2009 igem team

References