Difference between revisions of "Part:BBa K228867"
(No difference)

Revision as of 10:12, 22 September 2009


pSB1A2-lacZ screening plasmid

T7 polymerase with an amber mutation coding gene is constructed after AraC protein(reversed sequence) and Pbad promoter which would be triggered by Arabinose. T7 polymerase can work well in the exsitence of SupD tRNA. Besides, the expression of the T7 polymerase can be regulated by RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a suffix.
    Illegal SpeI site found at 2
    Illegal PstI site found at 303
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2345
    Illegal NheI site found at 14
    Illegal NheI site found at 37
    Illegal SpeI site found at 2
    Illegal PstI site found at 303
    Illegal NotI site found at 2351
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2345
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2345
    Plasmid lacks a suffix.
    Illegal SpeI site found at 2
    Illegal PstI site found at 303
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2345
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2360
    Illegal SpeI site found at 2
    Illegal PstI site found at 303
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1384