Difference between revisions of "Part:BBa K4361016"

 
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Similar to BBa_K4361015, but inserting an additional "tca + IR2" after the second IR1.
 
Similar to BBa_K4361015, but inserting an additional "tca + IR2" after the second IR1.
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===Usage and Biology===
 
  
 
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<partinfo>BBa_K4361016 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4361016 SequenceAndFeatures</partinfo>
  
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<h3>Usage and biology</h3>
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<html>
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<h3>Results</h3>
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~~~
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<figure>
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<a href="https://static.igem.wiki/teams/4361/wiki/parts/oligos-16.png"><img src="https://static.igem.wiki/teams/4361/wiki/parts/oligos-16.png" style="width:300px;margin-left:275px"></a>
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<figcaption> <b>Figure 2.</b> Results of the second Tapestation experiment, in which the fraction of DNA bound to BlcR was determined for different types of oligos. The first bar and bottom dashed line represent the results with </html>[[Part:BBa_K4361000]]<html> (scrambled oligo, negative control), the second bar and top dashed line correspond to those with </html>[[Part:BBa_K4361001]]<html> (wildtype oligo, positive control). The third bar depicts the measured fraction of bound DNA for this part.</figcaption>
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</figure>
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</html>
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 14:19, 8 October 2022


BlcR-binding oligo, 91 bp, IR1 + IR2 + IR1 + IR2

Similar to BBa_K4361015, but inserting an additional "tca + IR2" after the second IR1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and biology

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Results

~~~
Figure 2. Results of the second Tapestation experiment, in which the fraction of DNA bound to BlcR was determined for different types of oligos. The first bar and bottom dashed line represent the results with Part:BBa_K4361000 (scrambled oligo, negative control), the second bar and top dashed line correspond to those with Part:BBa_K4361001 (wildtype oligo, positive control). The third bar depicts the measured fraction of bound DNA for this part.