Difference between revisions of "Part:BBa K4361014"

 
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Based off of BBa_K4361001, but inserting the final 5 nucleotides of the 3' end (gcggg) before inverted repeat pair 2.
 
Based off of BBa_K4361001, but inserting the final 5 nucleotides of the 3' end (gcggg) before inverted repeat pair 2.
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4361014 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4361014 SequenceAndFeatures</partinfo>
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<h3>Usage and biology</h3>
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<html>
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<h3>Results</h3>
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~~~
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<figure>
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<a href="https://static.igem.wiki/teams/4361/wiki/parts/oligos-14.png"><img src="https://static.igem.wiki/teams/4361/wiki/parts/oligos-14.png" style="width:300px;margin-left:275px"></a>
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<figcaption> <b>Figure 2.</b> Results of the second Tapestation experiment, in which the fraction of DNA bound to BlcR was determined for different types of oligos. The first bar and bottom dashed line represent the results with </html>[[Part:BBa_K4361000]]<html> (scrambled oligo, negative control), the second bar and top dashed line correspond to those with </html>[[Part:BBa_K4361001]]<html> (wildtype oligo, positive control). The third bar depicts the measured fraction of bound DNA for this part.</figcaption>
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</figure>
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</html>
  
  

Revision as of 14:18, 8 October 2022


BlcR-binding oligo, 56 bp, IR1 + 5 bp linker + IR2

Based off of BBa_K4361001, but inserting the final 5 nucleotides of the 3' end (gcggg) before inverted repeat pair 2.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and biology

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Results

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Figure 2. Results of the second Tapestation experiment, in which the fraction of DNA bound to BlcR was determined for different types of oligos. The first bar and bottom dashed line represent the results with Part:BBa_K4361000 (scrambled oligo, negative control), the second bar and top dashed line correspond to those with Part:BBa_K4361001 (wildtype oligo, positive control). The third bar depicts the measured fraction of bound DNA for this part.