Difference between revisions of "Part:BBa K4345018"
 
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<partinfo>BBa_K4345018 short</partinfo>
 
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Design of the thermoregulated translation of toxin ccdB. Fluorescent protein sfGFP was linked to the antitoxin for visualisation.
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The toxin ccdB (including the stop codon control, see BBa_K4345018) is under the control of a promoter that is activated by both anhydrotetracyclin and lacI. ccdB is fused to sfGFP with a rigid linker.
  
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4345018 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4345018 SequenceAndFeatures</partinfo>
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===Functional Parameters===
 
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<partinfo>BBa_K4345018 parameters</partinfo>
 
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Latest revision as of 14:09, 8 October 2022


Tet/lac promoter - ccdB fused to sfGFP

The toxin ccdB (including the stop codon control, see BBa_K4345018) is under the control of a promoter that is activated by both anhydrotetracyclin and lacI. ccdB is fused to sfGFP with a rigid linker.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1265
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 352
    Illegal AgeI site found at 685
  • 1000
    COMPATIBLE WITH RFC[1000]