Difference between revisions of "Part:BBa K1031743"
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<p style="text-align:center"><b>Fig 1</b> Construction of biosensor circuit <i>Pa</i>/J23113-PaaX. The orange arrowheads represent promoters. RBS are shown in green ovals. The square in dark red stands for terminator B0015. | <p style="text-align:center"><b>Fig 1</b> Construction of biosensor circuit <i>Pa</i>/J23113-PaaX. The orange arrowheads represent promoters. RBS are shown in green ovals. The square in dark red stands for terminator B0015. | ||
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== '''Sequence and Features''' == | == '''Sequence and Features''' == | ||
Latest revision as of 13:49, 8 October 2022
J23113-PaaX
For detailed information concerning PaaX, please visit 2013 Peking iGEM Biosensor PaaX
Construction
In order to fine-tune the performance of PaaX biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031743 is composed of three elements, the constitutive Pc promoter J23113, coding sequence of PaaX and terminator B0015. (Fig 1)
Fig 1 Construction of biosensor circuit Pa/J23113-PaaX. The orange arrowheads represent promoters. RBS are shown in green ovals. The square in dark red stands for terminator B0015.
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]