Difference between revisions of "Part:BBa K4345012"

 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4345012 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4345012 SequenceAndFeatures</partinfo>
  
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===References===
  
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De Paepe, B., Maertens, J., Vanholme, B., & De Mey, M. (2018, April 24). Modularization and Response Curve Engineering of a Naringenin-Responsive Transcriptional Biosensor. ACS Synthetic Biology, 7(5), 1303–1314. https://doi.org/10.1021/acssynbio.7b00419
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===Functional Parameters===
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<partinfo>BBa_K4345012 parameters</partinfo>
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Revision as of 13:06, 8 October 2022


FdeR

Coding sequence of fdeR from PfdeAR-FdeR biosensor circuit's detector module. FdeR is a part of Naringenin-Responsive transcriptional biosensor develop by De Paepe et al. (2018). Native naringenin-responsive regulatory circuit, LysR-type PfdeAR-FdeR pair from Herbaspirillum seropedicae was redesigned to study the naringenin response in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 466
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 466
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 466
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 466
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 466
    Illegal NgoMIV site found at 653
    Illegal NgoMIV site found at 665
    Illegal NgoMIV site found at 756
  • 1000
    COMPATIBLE WITH RFC[1000]

References

De Paepe, B., Maertens, J., Vanholme, B., & De Mey, M. (2018, April 24). Modularization and Response Curve Engineering of a Naringenin-Responsive Transcriptional Biosensor. ACS Synthetic Biology, 7(5), 1303–1314. https://doi.org/10.1021/acssynbio.7b00419