Difference between revisions of "Part:BBa K4204004:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The sequence is designed to replace the promotor, RBS, and ORF of the original repressor. Thus it does not have a terminator and the original terminator of the repressor must be kept. Also, the promoter of the sequence might be shorter than marked, since the original research does not mark the promoter range clearly. | + | The sequence is designed to replace the promotor, RBS, and ORF of the original repressor. Thus it does not have a terminator and the original terminator of the repressor it replaced must be kept. Also, the promoter of the sequence might be shorter than marked, since the original research does not mark the promoter range clearly. |
| − | + | ||
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===Source=== | ===Source=== | ||
Revision as of 12:30, 8 October 2022
Transcriptional unit of EilR.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 475
Illegal AgeI site found at 625 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence is designed to replace the promotor, RBS, and ORF of the original repressor. Thus it does not have a terminator and the original terminator of the repressor it replaced must be kept. Also, the promoter of the sequence might be shorter than marked, since the original research does not mark the promoter range clearly.
Source
From research done by Ruegg et al [1]. Synthesized by twist bioscience.
References
[1] Ruegg, T. L., Pereira, J. H., Chen, J. C., DeGiovanni, A., Novichkov, P., Mutalik, V. K., Tomaleri, G. P., Singer, S. W., Hillson, N. J., Simmons, B. A., Adams, P. D., & Thelen, M. P. (2018). Jungle Express is a versatile repressor system for tight transcriptional control. Nature Communications, 9(1). https://doi.org/10.1038/s41467-018-05857-3