Difference between revisions of "Part:BBa K4152200"
Line 3: Line 3:
 
<partinfo>BBa_K4152200 short</partinfo>
 
<partinfo>BBa_K4152200 short</partinfo>
  
−
This composite part is made up of 7 basic parts: XhoⅠ, α-factor secretion signal, propeptide, PK_WT, His-Tag, Terminator, and EcoRⅠ. These elements are the target PK gene we constructed, which would be used to construct recombination pPIC9-PK.
+
These elements are the target PK gene we constructed, which would be used to insert the Proteinase K gene into pPIC9.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:10, 8 October 2022


XhoⅠ+Linker a+propeptide+PK_WT+His-Tag+Terminator+EcoRⅠ

These elements are the target PK gene we constructed, which would be used to insert the Proteinase K gene into pPIC9.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1159
    Illegal XbaI site found at 535
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1159
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1159
    Illegal XhoI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1159
    Illegal XbaI site found at 535
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1159
    Illegal XbaI site found at 535
  • 1000
    COMPATIBLE WITH RFC[1000]