Difference between revisions of "Part:BBa K4180000"
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<partinfo>BBa_K4180000 short</partinfo> | <partinfo>BBa_K4180000 short</partinfo> | ||
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| + | AMP is an energy sensor regulated by energy depletion, such as AMP (adenosine monophosphate): ATP (adenosine triphosphate) ration is high, AMPK is induced by upstream pathway to act as the master regulator of energy metabolism, it will increase catabolic process, such as triggering fatty acid catabolism for more ATP, and reduce anabolic process, such as protein and fatty acid synthesis to reduce energy consumed (Herzig and Shaw 2018)<br> | ||
| − | + | SNF1in yeast is homology of AMPKα in human, which is a major metabolism sensor protein of the whole metabolic pathways, increasing catabolic process, such as triggering fatty acid catabolism for more ATP, and reducing anabolic process, such as protein and fatty acid synthesis to reduce energy consumed <br><br> | |
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| + | Citation: Herzig, S. and R. J. Shaw (2018). "AMPK: guardian of metabolism and mitochondrial homeostasis." Nat Rev Mol Cell Biol 19(2): 121-135.<br><br> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
| − | + | </h4> | |
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| − | [[File:BBa K4180000.png|400px|thumb|left|<h3>     BBa_K4180000 SNF1 full length <br>          PCR product </h3>]] | + | [[File:BBa K4180000.png|400px|thumb|left|<h3>      BBa_K4180000 SNF1 full length <br>           PCR product </h3>]] |
| − | [[File:BBa K4180000-2.png|400px|thumb|left|<h3> | + | [[File:BBa K4180000-2.png|400px|thumb|left|<h3> After Bacteria transformation, bacteria colonies were picking up directly to do SNF1 full length PCR - colonies #1, 3, 5, and 6 had BBa_K4180000(SNF1           full length)<br>          PCR product </h3>]] |
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Latest revision as of 12:01, 8 October 2022
SNF1 is the metabolism sensor
AMP is an energy sensor regulated by energy depletion, such as AMP (adenosine monophosphate): ATP (adenosine triphosphate) ration is high, AMPK is induced by upstream pathway to act as the master regulator of energy metabolism, it will increase catabolic process, such as triggering fatty acid catabolism for more ATP, and reduce anabolic process, such as protein and fatty acid synthesis to reduce energy consumed (Herzig and Shaw 2018)
SNF1in yeast is homology of AMPKα in human, which is a major metabolism sensor protein of the whole metabolic pathways, increasing catabolic process, such as triggering fatty acid catabolism for more ATP, and reducing anabolic process, such as protein and fatty acid synthesis to reduce energy consumed
Citation: Herzig, S. and R. J. Shaw (2018). "AMPK: guardian of metabolism and mitochondrial homeostasis." Nat Rev Mol Cell Biol 19(2): 121-135.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 528
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]