Difference between revisions of "Part:BBa K4165240"
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Revision as of 10:01, 8 October 2022


His-UBC

his biobrick consists of T7 promotor (BBa_K3633015), RBS (BBa_K4165016), 6-His tag (BBa_K4165020), UBC (BBa_K4165014), T7 Terminator (BBa_K731721), The His tag was attached to the UBC coding sequence to serve in the purification using NI-NTA column.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 113
    Illegal BamHI site found at 82
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]